They added the beads to miRNAs isolated from cancer cell lysates and stained with streptavidin-phycoerythrin. When the samples were run through a flow cytometer, the instrument simultaneously ...
After being washed with phosphate-buffered saline, cells were incubated with 10 μl of 1:100 phycoerythrin-labeled goat anti-mouse IgM (eBioscience, USA) for 30 min at 4°C. After another wash, the ...
Flow Cytometry Sorting and Prostasphere Formation Assays Five to ten million of 22Rv1 and DU145 cells were stained with phycoerythrin (PE)- conjugated anti-CD44 and allophycocyanin-conjugated ...
1 Department of Clinical Immunology, University Hospital, Groningen, The Netherlands 2 Department of Rheumatology, University Hospital, Groningen, The Netherlands 3 Department of Pathology and ...
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